Fig 1.

Immunofluorescence detection of PPRV in Vero cells transfected with siNPPRV1. The cells were first transfected with 100, 33.3, 11.1, 3.7, and 0 nM siNPPRV1 (columns A to E, respectively). The day after, they were infected with Nigeria 75/1 vaccine virus (left panel) or with one of its escape mutants (si1mut1, right panel) at an MOI of 0.1 (row 1) and 10-fold serially diluted (rows 2 to 4). The presence of the virus was evidenced after 4 days of cell culture by a direct immunofluorescence test using a monoclonal antibody against the N protein coupled to TRITC. The left panel shows the dose/effect response with an RNAi-sensitive virus, while the right panel shows the results with a resistant virus.