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. 2012 Jan;86(2):1079–1089. doi: 10.1128/JVI.02662-10

Fig 7.

Fig 7

A deletion mutant of μNS lacking the minimal interaction domain (aa 222 to 271) still interacts with Hsc70. (A) Immunofluorescence images showing the distribution of the μNS(D271A) point mutant (left) and the μNS(Δ222-271) mutant (right). CV-1 cells were fixed and immunostained for μNS at 24 h posttransfection. Bars, 10 μm. (B) 293F cells were transfected with a plasmid encoding μNS(Δ222-271) (del) or full-length μNS (M3). Hsc70 was immunoprecipitated from cell lysates, immunoblotted, and probed with anti-Hsc70 and anti-μNS antibodies. (C) 293F cells were cotransfected with a plasmid encoding μNS(Δ222-271) (del) or full-length μNS (M3) together with the HA-tagged SBD of Hsc70 [Hsc70(395-650)]. HA-tagged proteins were immunoprecipitated from cell lysates, immunoblotted, and probed with anti-HA and anti-μNS antibodies.