Fig 6.

ICP0 interacts with tankyrase 1 and is sufficient to redistribute tankyrase 1 in ICP0-transfected cells. (A) HEp-2 cells were mock transfected (control) or transfected with plasmids carrying the ICP0 gene (HSV-1 or HSV-2). Cells were obtained using tankyrase 1 (green) and anti-Myc (red) antibodies. Merged images of the transfected cells are shown in the right panels. Imaging was performed using a Zeiss LSM 510 Meta confocal microscope with a 64× objective lens. Images were collected at a 3× digital zoom. (B) Western blotting. Transfected cells were collected at 12, 24, and 36 h posttransfection and analyzed by Western blotting using the indicated antibodies. (C) Coimmunoprecipitation assay. Transfected cells were collected at 24 h posttransfection, and whole-cell lysates (WCL) were immunoprecipitated (IP) with anti-Myc antibodies or subjected directly to Western blotting with the indicated antibodies. Normal rabbit IgG was used as a negative control for immunoprecipitation. Immunoprecipitated ICP0 was Western blotted using anti-Myc and HSV-1 ICP0 antibodies.