Table 2.

The α-helical content of N peptide alone and in mixtures with C34 or CP32 M^a

N peptide	[θ] at 222 nm (×103)	% Helicity	Tm (°C)
N36	−9.9	29.9	NAb
N36-Q64A	−7.3	22.2	NA
N36-Q64L	−8.5	25.9	NA
N36 + C34	−29.1	88.0	66
N36-Q64A + C34	−27.8	84.0	57
N36-Q64L + C34	−18.5	56.0	56
T21	−2.1	6.3	NA
T21-Q64A	−3.0	9.1	NA
T21-Q64L	−3.3	10.0	NA
T21 + CP32 M	−28.6	86.7	88.2
T21-Q64A + CP32 M	−23.3	70.6	82.0
T21-Q64L + CP32 M	−11.7	35.5	71.2

^aAll of the experiments were performed with 10 μM peptide solutions in phosphate-buffered saline (pH 7.0). The midpoint of thermal denaturation (T_m) was calculated from the thermal dependence of the CD signal at 222 nm.

^bNA, not applicable.