NAb responses to hexon- and fiber-chimeric Ad5 vectors. (A) Median log Ad5, Ad5KC68, Ad5HVR48, Ad5HVR48KC68, and Ad48 NAb titers in 72 C57BL/6 mice preimmunized with Ad5 are represented as box-and-whisker plots showing the full range, 25% to 75% interquartile range (box), and median (bar). ***, P < 0.0001; **, P = 0.0016. (B) Median log Ad5, Ad5KC68, Ad5HVR48, Ad5HVR48KC68, and Ad48 NAb titers in 267 South African serum samples are similarly represented as box-and-whisker plots. ***, P < 0.0001.