Fig 3.
Cellular immune responses induced by Ad5KC68 and Ad5HVR48KC68 vectors. Naïve (A to C) or Ad5-preimmunized (D to F) C57BL/6 mice (8/group) were immunized intramuscularly with 109 vp of the chimeric vectors Ad5, Ad5HVR48, Ad5KC68, and Ad5HVR48KC68 expressing SIV Gag. AL11-specific tetramer responses at multiple time points, IFN-γ ELISPOT assays using splenocytes from the mice on day 28 postimmunization, and ICS assays were utilized to measure SIV Gag-specific cellular immune responses. SFC, spot-forming cells. *, P < 0.05. Gag-specific antibodies were also assessed in naïve (G) or Ad5-preimmunized (H) mice (20/group) at weeks 0 and 4 by ELISA. * = P < 0.05.