Fig 3.

RRV trafficking is mediated by dynein. (A) Overexpression of dynamitin-GFP but not the enhanced green fluorescent protein (EGFP) vector efficiently inhibited RRV trafficking in RFs. Cells were transiently transfected with a dynamitin-GFP construct or a vector control. At 36 h posttransfection, cells were inoculated with RRV for 4 h and stained for RRV particles with a rabbit anti-RFP antibody. (B) Quantification of the total numbers of RRV particles docked at each nucleus. Images were acquired for 6 to 10 fields per coverslip to allow counting of 50 nuclei. Data were analyzed as described in the legend to Fig. 1. (C) Distribution of nuclei with different numbers of RRV particles.