Fig 6.

The integrity of NS2 is required for virion production. (Upper) N7/OsNS2-4A(H/A,C/A)(R1268Q,3′UTR) RNA transcripts harboring either a deletion of the C-terminal 10 codons of the NS2 gene (NS2delC10) or mutations in the putative Zn²⁺-binding motif in NS2 (C1500S and C1503S) were electroporated into MDBK cells. Expression of viral antigen was detected by IF at 24 h p.e. (Lower) Supernatants of these cells harvested at 48 h p.e. were subsequently used for infection of naïve MDBK cells, which were analyzed by IF 48 h p.i.