Absence of JNK affects the phosphorylation of both microtubule-associated protein (MAP-1B) and paxillin. (A to C) WT, JNK1/2-KO, and MKK4/7-KO cells were left uninfected or infected (MOI, 5). At 3, 6, 9, and 12 hpi, cell lysates were collected, subjected to Western blotting, and then probed with anti-phospho-MAP-1B (top) or anti-phospho-paxillin (middle) antibodies. (Bottom) Probing with anti-β-actin antibody as a loading control. The molecular masses (kDa) are indicated on the left. Data are representative of three independent experiments with similar results.