Fig 7.

Effect of RS-1 on HIV-1 replication and integration in HeLa P4 cells. (A) Effect of RS-1 treatment on hRAD51 DNA repair endogenous activity. The ability of RS-1 to promote cisplatin resistance was checked in a standard survival analysis after 24 h of treatment with increasing concentrations of the compound. (B) Effect of RS-1 treatment on HIV-1 replication in HeLa P4 cells. The effect of RS-1 on early steps of HIV-1 replication was measured by 4-MUG quantification of the β-galactosidase activity 48 h postinfection (MOI = 0.4) after 24 h of pretreatment with increasing RS-1 concentrations. (C) Effect of RS-1 on amount of two-LTR circles. The total and two-LTR circle viral DNA populations were quantified by quantitative PCR as described in Materials and Methods for two effective concentrations of RS-1. Data are reported as the ratios between the percentages of two-LTR circles and total DNA. (D) Effect of RS-1 on integrated viral DNA amount. The integrated DNA was amplified by specific PCR as reported in Materials and Methods and quantified on agarose gel (an example of amplification results obtained in the absence [w/o] or presence of 15 or 30 μM RS-1 treatment is shown). One hundred percent corresponds to the integrated DNA quantification obtained without RS-1 treatment. All results are the means from at least three representative independent experiments ± standard deviations (error bars). A Student test was performed on serial values. *, P < 0.05; **, P < 0.005.