Table 1.
DNA damage signaling in cells infected with HSV-1 or coinfected with HSV-1 and rAAV2a
| Protein | HSV-1–rAAV2 coinfection |
HSV-1 infection |
||||||
|---|---|---|---|---|---|---|---|---|
| Inhibition | Induction | Phosphorylation | Localization | Inhibition | Induction | Phosphorylation | Localization | |
| ATM | + | NUCc + RCs | + | RCs | ||||
| ATR | − | − | + | RCs | − | − | + | RCs |
| ATRIP | − | + | NDd | RCs | − | + | ND | RCs |
| DNA-PKcs | +b | − | + | RCs | + | − | − | − |
| USP7 | + | − | ND | RCs | + | − | ND | ND |
| H2AX | ND | ND | + | NUC | ND | ND | + | NUC |
| NBS1 | − | − | + | RCs | − | − | + | RCs |
| RPA32 (P-Ser4/8) | − | − | + | RCs | − | − | − | RCs |
| p53 | − | + | + | RCs | − | + | + | RCs |
| Chk1 | − | − | − | RCs | − | − | − | RCs |
| Chk2 | − | − | + | RCs | − | − | + | RCs |
a
Shown is a summary of data from WB analysis, IF analysis, and flow cytometry. Bold, AAV-induced modulation of DDR.
b
Inhibition delayed.
c
NUC, nucleus.
d
ND, not done.