Fig 2.

Time and temperature dependence of 19-kDa GP Ecto binding to liposomes. (A) 19-kDa GP Ecto was incubated with liposomes at pH 5.0 (black diamonds) or pH 7.5 (gray circle) at the indicated temperatures for 10 min, and the percentage of total 19-kDa GP Ecto bound to liposomes was measured as described in the legend to Fig. 1. Results of one of two experiments (triplicate samples) with similar results are shown. (B) Samples of 19-kDa GP Ecto and liposomes were incubated at pH 5.0 for the indicated times at 50°C, 42°C, or 37°C and processed as for panel A. Data are the averages from duplicate samples. Error bars indicate SD.