Fig 3.

Association of 19-kDa GP Ecto with liposomes is stable to treatment with 1 M NaCl or 2 M urea. The 19-kDa GP Ecto was incubated with liposomes at 50°C and pH 5.0 for 10 min and then processed as described in the legend to Fig. 1, except that after two washes with HM buffer, the liposome-bound beads were washed once with HM buffer containing 1 M NaCl or the indicated concentration of urea. The unbound, third-wash, and bound fractions were then analyzed for 19-kDa GP Ecto as described in the legend to Fig. 1. Data are the percentages of total GP1 detected in each fraction and are the averages from duplicate samples. Error bars indicate SD.