Fig 1.

Diagram of Rem and mutant constructs. The full-length Rem protein is depicted, with the white box representing the signal peptide and the hatched box representing the C-terminal domain. The inverted triangles indicate the positions of glycosylation sites (Gly) in the C terminus. The C-terminal truncation mutants are shown relative to their inclusion of Rem features. The domains indicated with horizontal double-headed arrows below MMTV-SP (SP) include the nuclear localization sequence (NLS)/arginine-rich motif (ARM) and the nuclear export sequence (NES). The relative position of mutations at amino acid 71 is also shown.