Fig 1.
rOC/ATCC and rOC/US183-241 induce programmed cell death in human neurons. Differentiated LA-N-5 cells were infected with rOC/ATCC or rOC/US183-241 for 24, 48, or 72 h. (A) Viability of infected human neurons. Cell viability was evaluated by the MTS-PMS assay and is expressed as relative percentage compared to that of mock-infected cells. (B) Fragmentation of DNA in infected neurons. Fragmented DNA was quantified by sandwich ELISA against histone and DNA and is expressed as a relative fold increase compared to that in mock-infected cells. (C) Fluorescence-activated cell sorting (FACS) TUNEL/7-AAD labeling of infected neurons. Cells stained with 7-AAD and TUNEL labeled were analyzed by FACS. The graph represent the percentage of quadrants. Nonsignificant percentages of 7-AAD-positive/TUNEL-negative cells were omitted. Statistical significance: *, P < 0.05; **, P < 0.01; ***, P < 0.001.