Fig 7.
Cyclophilin D inhibition alters AIF nuclear translocation in infected neurons. Differentiated LA-N-5 cells were infected with rOC/ATCC or rOC/US183-241 and treated with cyclosporine (CsA). (A) Detection of AIF by immunofluorescence. Cells were fixed and incubated with an anti-AIF antibody (green) and DRAQ5 (blue). Colocalization is represented by merged AIF and DRAQ5 signals (turquoise) as indicated by white arrows. (B) Western immunoblotting of mitochondrial AIF and CytC. Mitochondrial proteins fractions were subjected to Western immunoblotting analysis using antibodies directed against AIF or CytC. VDAC served as a loading control. Intensities of the bands were evaluated with ChemiGenius2 Syngene software and expressed as percentage relative to the loading control. Results are representative of two independent experiments.