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. 2012 Jan;86(1):36–48. doi: 10.1128/JVI.00717-11

Fig 1.

Fig 1

Nef-mediated down-modulation of human CD8αβ is conserved between primate lentiviruses. (A) Flow cytometric analysis of CEM cells expressing A2-CD8β fusions, SupT1 cells, and primary CD8+ T cells transduced with HIV-1 recombinants expressing EGFP alone or together with the indicated nef alleles. The ranges of EGFP expression used to calculate the MFI of A2-CD8β fusion or CD8αβ expression are indicated. (B) Correlations between the efficiencies of Nef-mediated down-modulation of CD8αβ in primary CD8+ T cells and of A2-CD8β fusions in CEM cells or CD8αβ in SupT1 cells. (C) Correlations between the efficiencies of Nef-mediated down-modulation of CD8αβ in primary CD8+ T cells and of CD28, CD4, and MHC-I in primary CD4+ T cells. Each symbol represents average n-fold down-modulation (n = 3) of the indicated receptor molecule by one individual nef allele analyzed. An overview of the HIV and SIV nef alleles used is provided in Table S1 in the supplemental material.