Fig 6.

Functional analysis of SIVagmSab Nef mutants. (A) The SIVagmSab Nef amino acid sequence is given in single-letter code. Mutated sequence motifs are highlighted by blue boxes, and positions of alanines are indicated. (B) CEM cells expressing A2-CD8β fusions or SupT1 cells were transduced with HIV-1 recombinants expressing EGFP alone or together with the indicated nef alleles and were assayed for surface expression of the A2-CD8β fusion, CD8αβ, CD4, CD28, and MHC-I. Quantification was performed as described in Materials and Methods. Data shown are average values ± SD derived from two independent experiments.