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Diagram of sequential immunoprecipitation. Healthy or VACV-infected T2-B27 transfectant cells (4 × 1010) were lysed. HLA-peptide complexes were isolated via affinity chromatography of the soluble fraction of cell extracts with the following mAbs, used sequentially: PA2.1 (anti-HLA-A2), ME1 (anti-HLA-B27), and W6/32 (specific for a monomorphic HLA class I determinant).
