Fig 6.

In vivo killing activity of VACV peptide-specific CTLs in HLA-A*0201-transgenic mice. HLA-A*0201-transgenic mice were infected i.p. with VAVC. One week later, they were injected i.p. with syngeneic splenocytes that were either labeled with a low level of CFSE and not subjected to pulsing or pulsed with the indicated VACV peptides and labeled with a high level of CFSE. Two days later, the remaining CFSE-labeled target cells in the peritoneal cavity were lavaged and analyzed by flow cytometry. Percent in vivo lysis values for individual animals (circles) ± SD (lines) are shown.