Fig 7.
Recognition by CTLs of VACV epitopes presented by the HLA-A*0201 class I molecule. (A to C) RMA-HLA-A*0201 target cells prepulsed with 10−5 M A17L9-17 (A), A10L614-623 (B), and A10L688-696 (C) synthetic peptides (filled squares) were tested in a cytolytic assay with their respective memory peptide-specific CTL lines obtained from HLA-A*0201-transgenic mice immunized up to 30 days before with VACV (upper panels). Negative controls used were unpulsed cells (open squares) and pulsed cells with the KPNA2 peptide (open circles). The data represent the means of the results of at least three independent experiments. (D) HLA-A*0201 TAP+ cells were infected with VACV (open bars) or not (filled bars) at a multiplicity of infection of 40 PFU/cell and analyzed by ICS for CD8+ T cell activation with the HLA-A*0201 memory peptide-specific CTL lines. The data represent the means of the results of 4 to 6 independent experiments.