Fig 1.

Mapping of the VP16 region involved in VP1/2 binding. (A) A schematic representation of GST-tagged VP16 truncation mutants and summary of results, indicating the GST-VP16 truncation mutants that bound very strongly (++++), strongly (+++), moderately (++), or weakly (+) or did not bind (−) to GFP-VP1/2NT. See Materials and Methods for details of quantification. FL, full-length. (B and C) 293T cells were cotransfected with GFP-VP1/2NT and the indicated GST-VP16 truncation mutants. Cell lysates were harvested at 48 h posttransfection and incubated with glutathione-Sepharose beads. Both cell lysates and bound protein complexes (GST pulldowns) were separated by SDS-PAGE and analyzed by Western blotting with anti-GST and anti-GFP antibodies. α, anti.