Fig 4.

K343A mutation in VP16 moderately inhibits virus replication. HaCaT cells were infected with the indicated viruses and harvested at various time points. (A and B) Total infectious virus yields were determined by plaque assay on Vero cells. The titers represent mean PFU/ml, and error bars represent standard errors of the means of triplicate samples. (C and D) Cell lysates were separated by SDS-PAGE and analyzed by Western blotting using the indicated antibodies. Tubulin was included as a loading control.