Fig 5.

VP16(K343A) is incorporated into virions in amounts similar to those of wt VP16. Extracellular virions were purified from the medium of infected HaCaT cells by Ficoll density gradient centrifugation, and VP5 levels in a range of PFU concentrations were analyzed by Western blotting. The appropriate PFU amounts to give equivalent levels of VP5 for each purified virion preparation were separated by SDS-PAGE and analyzed by Western blotting using the indicated antibodies. Numbers on the left represent molecular weight markers.