Table 2.
GST-tagged VP16 truncation constructs
| Plasmida | Primers used |
|---|---|
| GST-VP16 (FL) | COL281 + COL282 |
| GST-VP16(1-411) | COL281 + COL307 |
| GST-VP16(1-387) | COL281 + SS29 |
| GST-VP16(1-369) | COL281 + SS28 |
| GST-VP16(1-359) | COL281 + SS44 |
| GST-VP16(1-348) | COL281 + SS27 |
| GST-VP16(1-337) | COL281 + SS45 |
| GST-VP16(1-327) | COL281 + SS26 |
| GST-VP16(1-314) | COL281 + SS46 |
| GST-VP16(1-305) | COL281 + SS30 |
| GST-VP16(1-206) | COL281 + SS20 |
| GST-VP16(1-106) | COL281 + SS19 |
| GST-VP16(299-411) | SS24 + SS25 |
| GST-VP16(322-411) | SS33 + SS25 |
| GST-VP16(332-411) | SS34 + SS25 |
| GST-VP16(342-411) | SS35 + SS25 |
| GST-VP16(354-411) | SS36 + SS25 |
| GST-VP16(364-411) | SS37 + SS25 |
| GST-VP16(332-348) | SS34 + SS27 |
a
Numbers in parentheses represent amino acids of VP16 encoded. FL, full-length.