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VP16 mutants created by site-directed mutagenesis in the context of GST-VP16(1-411)
| Amino acid substitution(s) | Designated plasmid namea | Primers used |
|---|---|---|
| S333A G334A | M1 | SS54 + SS55 |
| Y335A F336A M337A | M2 | SS56 + SS57 |
| V338A L339A I340A | M3 | SS58 + SS59 |
| R341A K343A | M4 | SS60 + SS61 |
| L344A D345A S346A | M5 | SS62 + SS63 |
| Y347A S348A S349A | M6 | SS64 + SS65 |
| R341A | NA | SS74 + SS75 |
| K343A | NA | SS76 + SS77 |
| K343R | NA | SS102 + SS103 |
| K343M | NA | SS104 + SS105 |
| K343Q | NA | SS106 + SS107 |
| K343H | NA | SS108 + SS109 |
NA, not applicable
