Fig 2.
Resting human CD4+ T cells are present in BLT mice. (A) Flow cytometry analysis of pooled BLT mouse mononuclear cells prior to negative selection showed the presence of CD4+ and CD8+ T cells. Within the CD4+ T cell population, a heterogeneous population of cells expressing variable levels of CD27, CD25, HLA-DR, and CD11b was observed. (B) Following negative selection, flow cytometry analysis showed a homogeneous population of human CD4+ CCR7+ CD27+ CD8− CD25− HLA-DR− CD11b− resting T cells. Results for one representative BLT mouse of two are shown.