Fig 5.

Binding of peptides to Sf9 insect cells. (A) Sf9 cells were incubated with FITC-labeled peptides in insect culture medium (pH 6.2) at 4°C to prevent endocytosis. Five hours later, cells were washed and observed under a fluorescence microscope. (B) Sf9 cells were incubated with FITC-labeled GT24 in insect culture medium (pH 6.2) at 27°C to allow endocytosis. Two hours later, cells were washed and observed under a fluorescence microscope with a 20× objective. The right panel shows the enlarged image of the area marked by the yellow rectangle in the left panel. Note the multiple green fluorescent vesicles inside cells.