Fig 1.

Production of IFNs in primary cultured kidney cells prepared from RIG-I- and MDA5-deficient mice. Kidney cells were pretreated with 100 U of IFN-β for 2 h and infected with PV at an MOI of 10. RNA was prepared from the infected cells at 6 hpi. The amounts of IFN-α mRNA (A) and IFN-β mRNA (B) were determined using quantitative real-time PCR. Cells were prepared in duplicate, and the experiments were repeated three times. Representative data are shown. The amount of IFN activity in the supernatant of infected kidney cells at 8 hpi was determined by the cytopathic effect dye uptake method using L929 cells (C). ND, not detected.