Fig 3.

Ablation of TbPPR9 abolishes OXPHOS. (A) Representative growth curve of tetracycline-uninduced (−Tet) and -induced (+Tet) representative clonal T. brucei TbPPR9 RNAi cell line in glucose-free SDM-80 culture medium. The cross indicates that further incubation led to the death of the whole population. (B) In organello mitochondrial ATP production induced by succinate, α-ketoglutarate, and pyruvate of a tetracycline-uninduced (−Tet) and -induced (+Tet) TbPPR9 cell line was determined using luciferase. The substrates tested and the additions of antimycin (Antim.) and atractyloside (Atract.) are indicated at the top and the bottom, respectively. ATP production in mitochondria isolated from uninduced cells tested without antimycin or atractyloside is set to 100%. The bars represent means expressed as percentages. Standard errors of three independent biological replicates are indicated.