Fig 5.

RT-PCR analysis of the edited COX3 mRNA. Primers designed to preferentially amplify the fully edited COX3 mRNA were used for RT-PCR of RNA samples that were isolated from uninduced cells and from TbPPR9 RNAi cells that were induced for 48 and 120 h. The Northern blots detecting the TrpRS1 mRNA in Fig. 4 were used to normalize the concentration of the RNA samples that were used for the RT-PCR analysis. After 15, 25, and 35 cycles, aliquots were analyzed on an agarose gel and stained with ethidium bromide. All reactions were performed in the presence (+RT) and absence (−RT) of reverse transcriptase. The expected length of the cDNA corresponding to the fully edited COX3 mRNA of 654 nucleotides is indicated by the arrow.