Fig 6.

Lack of TbPPR9 affects the COX complex but not the cytochrome bc₁ or the ATP synthase complex. Total protein from the uninduced and induced TbPPR9 RNAi cell line (days of induction are indicated at the top) was analyzed for the levels of Ty1-tagged TbPPR9 (PPR9-Ty1), COX4 of the COX complex, cytochrome c₁ (CYT c1) of the cytochrome bc₁ complex, and the F1β subunit of the ATP synthase complex (F1β) using immunoblots and specific antisera. Each signal was normalized to the levels of cytosolic translation elongation factor 1a (EF-1a) to correct for loading differences. The graph shows a quantification of the normalized signals. The level of the proteins in cells induced for 1 day was set to 100%.