Fig 7.

TbPPR9 is a component of a large soluble complex. (A) Mitochondria of a cell line expressing HA-tagged TbPPR9 were fractionated into membrane (Mem) and matrix (Mat) fractions. (Top) Immunoblot stained with the HA antiserum; (middle) immunoblot analysis for the outer membrane protein VDAC, which serves as a membrane marker; (bottom) an ethidium bromide-stained agarose gel containing RNA isolated from the three fractions; only the region corresponding to tRNAs that serve as a matrix marker is shown. (B) Mitochondrial digitonin extracts from cell lines expressing HA-tagged TbPPR2, -3, -5 (29), and -9 as well as from a cell line expressing c-myc-tagged mitochondrial large-subunit ribosomal protein 21 (MRPL21) (29) were resolved on the same blue native gel, blotted, and analyzed using anti-HA and anti-c-myc antisera, respectively.