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. 2012 Jan;11(1):42–52. doi: 10.1128/EC.05250-11

Fig 2.

Fig 2

Effect of CaCdc53 neddylation on C. albicans morphology. (A) Sequence alignment of C. albicans Rub1 with human NEDD8 and S. cerevisiae Rub1. (B) CaCdc53 is unneddylated in the Carub1−/− strain and hyperneddylated in the Cajab1−/− strain. One CaCDC53 allele in strains KC2, KC208, and KC213 was fused to the 3× HA epitope tag by using plasmid KB1570. Proteins were extracted from cells in log phase, electrophoresed, and incubated with the 12CA5 antibody. (C) Cell morphology of the wild-type (WT) (KC2), Carub1−/− (KC208), and Cajab1−/− (KC213) strains grown to log phase at 30°C. Magnification, ×40. Bar = 10 μm. (D) Phenotype of CaCDC53RRR mutant. (Left) Sequence alignment of the neddylation sites of hCul1, SpPcu1, and CaCdc53. The arrowhead indicates the site of neddylation in SpPcu1 and HsCul1. The lysine residues that were mutated to arginine in CaCdc53 are underlined. (Right) Micrographs of the CaCDC53+/− (KC326) and CaCDC53RRR/− (KC327) strains grown to log phase at 30°C. Magnification, ×40. Bar = 10 μm.