Fig 5.
Stability of the F-box protein CaGrr1. A construct carrying 6× Myc-tagged CaGRR1 under the control of its own promoter (plasmid KB1799) was integrated into the same strains as those used for Fig. 4 and also into KC363 (Cacdc53-1). (A) Protein extracts from log-phase cells were Western blotted and sequentially incubated with anti-Myc (antibody 9E10; upper panel) and anti-β-actin (lower panel). Two independent transformants of the wild-type strain were tested. The numbers below the blots give the ratio of the Myc signal to the actin signal, normalized to the ratio for the wild-type strain (average for the two wild-type cultures; the variance is indicated). (B) [35S]methionine pulse-chase analysis of the same strains. C, no-tag control.