Fig 6.

Inhibition of erythrocyte binding to DBPII-SalI expressed on the surface of COS7 cells by rat anti-SalI and anti-DEKnull. A monolayer culture of COS7 cells expressing recombinant DBPII-SalI on the cell surface was tested for binding to Duffy blood group antigen on human erythrocytes in the presence of anti-DEKnull and anti-SalI sera. Antibodies were tested by endpoint dilution, and percent inhibition was quantified by comparing the number of rosettes bound to COS7 cells in 30 microscope fields (×200) in the presence of test sera and preimmune sera. Curves represent means of three independent experiments done in duplicate. Error bars represent ±standard deviation.