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. 2012 Jan 9;196(1):29–36. doi: 10.1083/jcb.201105009

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© 2012 Howitt et al.

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Figure 3. — Ndfip1 regulates PTEN nuclear import. (A) Nuclear FRAP analysis of cherry-PTEN shows enhanced transport upon coexpression of Ndfip1-GFP after photobleaching. Errors (SEM) are shown as shaded areas. (B) Images of typical cells from A at pre- or postbleach times and at experimental end points. Relative time points are indicated. (C) Nuclear FRAP analysis of GFP-PTEN shows the absence of transport in Ndfip1^−/− MEFs compared with Ndfip1^+/+ MEFs. Errors (SEM) are shown as shaded areas. (D) Images of typical cells from C at pre- or postbleach times and at experimental end points. Relative time points are indicated. (B and D) Bars, 10 µm. (E) Quantification of FRAP analyses after expression of cherry-PTEN (cPTEN) alone, with GFP (+GFP) or Ndfip1-GFP (+Ndfip1), or in the indicated cell type (cPTEN, n = 11; GFP, n = 4; Ndfip1, n = 5; Ndfip1^+/+ and Ndfip1^−/− MEF experiments, n = 10). Values are mean ± SEM.