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. 2012 Jan 9;196(1):103–114. doi: 10.1083/jcb.201105127

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© 2012 Gilden et al.

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Figure 4. — Septin aggregates are independent of ion transport. (a and b) Treatment with ion channel inhibitors charybdotoxin (Char.) and NPPB did not influence initial or maximum volume of control (Ctl.) or SEPT7KD cells in the flow cytometry osmotic swelling assay, but both drugs significantly inhibited RVD. Data are pooled from three independent experiments. Error bars represent SD. AU, arbitrary unit. (c) Single-plane time-lapse images of SEPT6-GFP–expressing control cells or cells treated with charybdotoxin or NPPB demonstrate that swelling-induced septin assembly on the cortex can occur in the absence of volume recovery. The bottom row of images taken 20 min after a media change are 3D reconstructions of whole cells. Bar, 10 µm.