Figure 7.

Dynein-dependent and -independent chromosome mobility contributes to homologue pairing in early meiotic nuclei. (A) To visualize the search radius of PCs under different conditions, 40 GFP::HIM-8 tracks from WT TZ, WT premeiotic, or TZ in dynein knockdown (dlc-1 RNAi in dhc-1(or195ts)) nuclei were rotated about a 3.5-µm-diameter sphere such that all tracks initiate at the same point. (B) RMSD plots for all GFP::HIM-8 trajectories in WT TZ nuclei, the same data with PCMs computationally removed, in TZ nuclei from dynein knockdown animals, and in premeiotic nuclei. (C) Example of a simulated collision between two objects traveling in random walks on the surface of a sphere. The star indicates the point at which the objects come within 200 nm at the same time point. (D) Comparison of time required to achieve collision in 95% of simulations based on motion parameters measured for the indicated conditions. Collision times were calculated from ≥10 independent sets of 100 simulations; error bars indicate the SD for all sets. See Materials and methods for details.