Table 2.
Primers used in this studya
| Primer | Sequence (5′ to 3′)a | Comment |
|---|---|---|
| MexCforEcoRI | CATGAATTCTCCTCGAAGCGCTTCCGCACGACGATG | |
| MexCrevHindIII | CATAAGCTTGTGAGGACTGATCTTCCCGAGTG | |
| mutSforEcoRI | GATGAATTCGAGCTGCTGATTCCAGACGACTG | |
| mutSrevBamHI | GATGGATCCGAACGGTGTCTCCAGCACCTGC | |
| mutSfor2 | CGTGCTGGACATCACCAGCG | |
| mutSrev2 | GAAGCTGCCGATGTGCGCAAGCAGCAC | |
| lpxCfor | CATGAATTCGAGATCGTCGGCAATCCACGCCTG | |
| lpxCrev | CATGAATTCCAGGAGTAGAGATGTGATTGGTG | |
| lpxCQCfor | GATCATGGCTTTGGCCTCTTAAGCGCTGACTGCG | |
| lpxCQCrev | CGCAGTCAGCGCTTAAGAGGCCAAAGCCATGATC | |
| attB1 | GGGGACAAGTTTGTACAAAAAAGCAGGCT | Gateway adapter |
| attB2 | GGGGACCACTTTGTACAAGAAAGCTGGGT | Gateway adapter |
| attB1-02 | GGGGACAAGTTTGTACAAAAAAGCAGGCTCGAGGAGGATATTC | Gateway adapter for expression constructs |
| attB2-02 | GGGGACCACTTTGTACAAGAAAGCTGGGTTTCA | Gateway adapter for expression constructs |
| lpxC 5′ | TACAAAAAAGCAGGCTCAGGAGTAGAGATGTGATTGGTG | lpxC cloning |
| lpxC 3′ | TACAAGAAAGCTGGGTGAGATCGTCGGCAATCCACGCCTG | lpxC cloning |
| lpxC US | CAACCAGTCTCACGGCAG | Amplify lpxC; 200-bp upstream region |
| 5′ fabG | TCGAGGAGGATATTCATGAGTCTGCAAGGTAAGGTCG | fabG cloning |
| 3′ fabG | CAAGAAAGCTGGGTTTCATCAGCTCATGTACATCCCACCATTG | fabG cloning |
| PaLpxCNdeIFP | GGGAATTCCATATGATCAAACAACGCACCTTGAAG | |
| PaLpxCXhoIRP | CCGCTCGAGCTACACTGCCGCC |
a
Restriction sites are in boldface.