Table 5.
Decrease in susceptibility of P. aeruginosa strains to CHIR-090 upon passaging in CHIR-090
| Passage | CHIR-090 susceptibilitya (μg/ml) |
||
|---|---|---|---|
| K767 | K2153 | CDR0017 | |
| 0 | 1 | 1 | 1 |
| 1 | 4 | 4 | 8 |
| 2 | 4 | 4 | 16 |
| 3 | 8 | 16 | 64 |
| 4 | 8 | 16 | >128 |
| 5 | 16 | 32 | >128b |
| 6 | 32 | 32 | ND |
| 7 | 32 | 64 | ND |
| 8 | 64 | 64 | ND |
| 9 | 64 | 64 | ND |
| 10 | 128 | 128 | ND |
a
Indicated as susceptibility rather than MIC. This is a measure of the susceptibility of the mixed population determined using a nonstandardized inoculum from the previous passage as described in Materials and Methods. Examples of isolated colonies from each step had susceptibilities within one dilution step of that reported in the table, determined with standard MIC testing. ND, not done.
b
Colonies were isolated from this well for Western blot analysis (Fig. 3).