Table 2.
MICs of BM212 and amino acid substitutions in the mmpL3 gene of M. smegmatis mutants resistant to BM212a
| M. smegmatis strain(s) | MIC (μg/ml) | Amino acid change (position) | Mutation(s) in another M. smegmatis ORF(s) |
|---|---|---|---|
| mc2155 (wt) | 3.125 | ||
| MSMEG-GR12.5 | 12.5 | A254V | |
| I296L | |||
| MSMEG-BM1, MSMEG-BM2, MSMEG-BM7, MSMEG-BM8 | 12.5 | I249T | |
| MSMEG-BM3, MSMEG-BM4 | 12.5 | F240L | |
| MSMEG-BM6 | 12.5 | L196P | |
| MSMEG-BM9 | 12.5 | A347V | |
| MSMEG-BM10 | 12.5 | V689G | |
| SRBM212_20 | 25 | A326T | |
| SRBM212_21 | 25 | A326T | MSMEG_2883, T130T |
| SRBM212_24 | 25 | V689G | MSMEG_0931c, +C; 3682514, T>C (noncoding); deletion of MSMEG_1716–MSMEG_1726 |
| SRBM212_26 | 25 | V197M |
a
The different nomenclatures of strains refer to mutants isolated during different experiments (see Materials and Methods). wt, wild type.