Table 3.
MICs of BM212 and amino acid substitutions in the mmpL3 genes of M. bovis BCG and M. tuberculosis H37Rv mutants resistant to BM212a
| Strain(s) | MIC (μg/ml) | Amino acid change (position) |
|---|---|---|
| M. bovis | ||
| BCG (wt) | 0.78 | |
| BCG-BM40 | 6.25 | I250M |
| L320P | ||
| BCG-BM41 | 6.25 | T286K |
| L320P | ||
| BCG-BM42, BCG-BM43, BCG-BM49, BCG-BM52–BCG-BM56 | 3.12 | L320P |
| BCG-BM44 | 3.12 | T277M |
| L320P | ||
| BCG-BM50 | 3.12 | A316T |
| L320P | ||
| BCG-BM51 | 3.12 | A294T |
| L320P | ||
| BCGRBM212_1 | 20 | L320P |
| BCGRBM212_2 | 20 | V240A |
| M. tuberculosis | ||
| H37Rv (wt) | 1.5 | |
| TBRBM212_1 | 25 | L215S |
a
The different nomenclatures of strains refer to mutants isolated during different experiments (see Materials and Methods).