Table 1.
Deferred-antagonism assay analysis of the protective capabilities conferred by NsuFEG and SpiFEG when expressed in L. lactis HP, or the resistance of the natural S. infantarius isolate, against the action of a range of natural nisin variant producersa
| Nisin variant | Avg zone size (mm) ± SD |
|||
|---|---|---|---|---|
| HP (control) | HP pNZ44nsuFEG | HP pNZ44spiFEG | S. infantarius | |
| A | 18.6 ± 0.71 | 16.3 ± 0.71 | 17.85 ± 1.06 | 2.94 ± 0.18 |
| F | 20.6 ± 1.00 | 17.79 ± 0.27 | 19.67 ± 1.00 | 5.84 ± 0.50 |
| Z | 24.4 ± 0.36 | 20.2 ± 0.14 | 21.85 ± 1.62 | 4.9 ± 0.74 |
| Q | 16.08 ± 0.65 | 14.8 ± 0.28 | 15.05 ± 1.62 | 4.17 ± 0.75 |
| U | 8.4 ± 0.42 | 0 | 4.7 ± 1.27 | 0b |
| U3 | 16.06 ± 0.18 | 0 | 12.2 ± 2.97 | 0b |
a
Values are averages of triplicate experiments and represent zone sizes, i.e., diameter of zone minus diameter of bacterial growth.
b
No distinct zone but some hazy growth adjacent to nisin-producing colony.