Fig 3.

Binding of DPX to whole parent and mutant cells. Bacterial cells were treated with increasing concentrations of DPX, and fluorescence was determined by emission at 485 nm upon excitation at 340 nm. ■, B. multivorans ATCC 17616; □, B. multivorans 26D7; ◊, B. multivorans RMI19; ▴, P. aeruginosa ATCC 27853. The background fluorescence of DPX with buffer alone was subtracted at each concentration. The data represent the means for three biological replicates ± standard errors of the means.