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Primers and probes for the quantification of qnr genes
| Primer or probe | Sequence (5′ → 3′)a | Target | Amplicon length (bp) |
|---|---|---|---|
| Primers | |||
| QnrA1-F | CTTCCTTGATGGGCTCAGATC | qnrA | 375 |
| QnrA1-R | CCAGATCGGCAAAGGTTAGG | ||
| QnrB1-F | GTAGCGCATATATCACGAATACC | qnrB | 481 |
| QnrB1-R | AGATCTGAACCACTGAACGTC | ||
| Probes | |||
| QnrA | FAM-CAGCCCCGCAGATTGACCTGTTG-BHQ1 | qnrA | |
| QnrB | FAM-ACCTGGGCACCTATCCAACGGTTT-BHQ1 | qnrB |
FAM, 6-carboxyfluorescein (a fluorescence reporter dye); BHQ, Black Hole Quencher.
