Fig 1.

Design of second-generation shRNAs for HIV-1 D30N and L90M variants. (A) Resistance of the D30N and L90M viruses against nelfinavir (NFV) and saquinvir (SQV). Fitness was compared to that of the wt virus. These data were derived from previous publications (13, 18, 28). (B) Position of the D30N and L90M mutations in the HIV-1 genome. The exact A residue that is mutated to G in the D30N or L90M variant is marked in black. (C) The expression cassettes of shRNA-D30N and shRNA-L90M under the control of the polymerase III promoters H1 and U6, respectively. Both expression cassettes were combined in shRNA-combi.