Fig 6.

Mixed infection of SupT1 cells expressing second-generation shRNAs under PI pressure. SupT1 cells that express the indicated shRNA inhibitor were infected with a mixture of wt-D30N-L90M viruses at the ratio of 10:1:1. These cultures were maintained without drugs or with SQV, NVF at its IC₃₀, and NVF at its IC₉₀. Cultures expressing no shRNA (JS1) served as a positive control. Virus replication was monitored over time by measuring CA-p24 concentration in the culture supernatant using ELISA. After a second round of infection, the provirus was sequenced to determine the most dominant virus variant present in the culture. These results were plotted in Table 1.