Fig 2.
Combination experiment strategy. Compound A was dispensed in all wells of column 1 and then 2.5-fold serial diluted down the row (landscape plate) from left to right until column 16, each in duplicate. Compound B was dispensed in wells A1 to A16 and then 2.5-fold serial diluted from top to bottom from row A through P, each in duplicate. Compounds A and B also were 2.5-fold serial diluted in columns 17 and 19 (landscape plate) and in columns 18 and 20 (portrait plate) to determine their individual IC50s. Controls included the following columns: 22, 100% DMSO as a negative control (0% growth inhibition); 23, 10 mM mefloquine solution as a positive control (100% growth inhibition); 21 and 24, 3-fold serial dilutions of 10 mM mefloquine and 10 mM chloroquine, respectively. Binary combinations were performed by combining a portrait plate (compound A) and a landscape plate (compound B) in a 384-well microplate. Additional controls included combining a landscape plate with its portrait counterpart.