Fig. 4.
AβPP expression was not affected by GLT-1 heterozygousity. Western blot analysis of protein from the cortex of 4 mice of each genotype at 6 months (A) or 9 months (B). The upper panels represent levels of membrane-associated AβPP. Full-length AβPP was detected using 22C11, 6E10, and anti-AβPP C-terminus (AβPP-CT) antibodies. AβPP-CT also detected AβPP C-terminal fragments (α and β AβPP-CTFs). C) Levels of secreted sAβPPα in 6- or 9-month-old mice were detected using the 6E10 antibody. Western blots were stripped and re-probed with an antibody recognizing actin.